ログイン 概要 よくある質問
1
1

過去の進歩の歴史を後程アップしておきます。

質問日 Feb 25 '11 at 15:05

okubo's gravatar image

okubo
1312913

edited Feb 25 '11 at 19:34

mn3's gravatar image

mn3 ♦♦
5154922


Here's a list George Church uses, I think.

  1. Illumina-GA SbP Fluorescent read-length 2*110 bp
  2. AB-SOLiD SbL Longest ligation reads
  3. CGI SbL $2000 genome, rolony grid, 100Kb haplotypes
  4. Polonator SbL/P Open-source, $170K device, 100Mb haplotypes
  5. Roche-454 SbP Long reads (>0.4 kb)
  6. Helicos SbP-sm Hi h parallelism & quantitation
  7. Ion Torrent SbP $50K, small device
  8. Pacific Bio SbP-sm Long reads (>2.0 kb) 9 Intelligent Bio SbP hexagonal grid
  9. Halcyon EM-sm Long reads (>Mb), $100 genome
  10. Genizon BioSci SbH in situ sequencing
  11. LightSpeed SbL 16X higher density >10X speed
  12. Bionanomatrix SbP-sm Fluorescent mapping
  13. OxfordNanopore Pore-protein-sm small device
  14. Visigen SbP-sm Pol <> dNTP FRET
  15. ZS Genetics EM-sm Iodine labels
  16. Nabsys Pore-SbH-sm small device
  17. GE Global SbP-sm
  18. IBM Pore Si-sm small device
  19. Electronic Biosci Pore-protein-sm
  20. GnuBio SbP-picoliter droplets

The few additions are: CGI Polonator (it's a company?) Roche-454 Ion Torrent GeniZon BioSci GE Global IBM Electroni Biosci

回答日 Mar 01 '11 at 00:34

okubo's gravatar image

okubo
1312913

http://seqanswers.com/forums/archive/index.php

Single-Molecule DNA Sequencing of a Viral Genome

Timothy D. Harris,1* Phillip R. Buzby,1 Hazen Babcock,1 Eric Beer,1 Jayson Bowers,1 Ido Braslavsky,2 Marie Causey,1 Jennifer Colonell,1 James DiMeo,1 J. William Efcavitch,1 Eldar Giladi,1 Jaime Gill,1 John Healy,1 Mirna Jarosz,1 Dan Lapen,1 Keith Moulton,1 Stephen R. Quake,3 Kathleen Steinmann,1 Edward Thayer,1 Anastasia Tyurina,1 Rebecca Ward,1 Howard Weiss,1 Zheng Xie1

The full promise of human genomics will be realized only when the genomes of thousands of individuals can be sequenced for comparative analysis. A reference sequence enables the use of short read length. We report an amplification-free method for determining the nucleotide sequence of more than 280,000 individual DNA molecules simultaneously. A DNA polymerase adds labeled nucleotides to surface-immobilized primer-template duplexes in stepwise fashion, and the asynchronous growth of individual DNA molecules was monitored by fluorescence imaging. Read lengths of >25 bases and equivalent phred software program quality scores approaching 30 were achieved. We used this method to sequence the M13 virus to an average depth of >150x and with 100% coverage; thus, we resequenced the M13 genome with high-sensitivity mutation detection. This demonstrates a strategy for high-throughput low-cost resequencing.

1 Helicos BioSciences Corporation, One Kendall Square, Cambridge, MA 02139, USA. 2 Department of Physics and Astronomy, Ohio University, Athens, OH 45701, USA. 3 Department of Bioengineering, Stanford University, and Howard Hughes Medical Institute, Stanford, CA 94305, USA. The paper abstract and full text (for subscribers) is located here: http://www.sciencemag.org/cgi/content/abstract/320/5872/106

回答日 Mar 01 '11 at 00:38

okubo's gravatar image

okubo
1312913

edited Mar 01 '11 at 00:50

あなたの回答
プレビューをトグルする

この質問をフォローする

By Email:

Once you sign in you will be able to subscribe for any updates here

By RSS:

回答

回答とコメント

タグ:

×47
×13
×13

質問日: Feb 25 '11 at 15:05

閲覧数: 8,136 回

最終更新日: Mar 01 '11 at 00:50

powered by OSQA